Cytoplasmic interaction of LysM receptors contributes to the formation of symbiotic receptor complex.
Identifieur interne : 000185 ( Main/Exploration ); précédent : 000184; suivant : 000186Cytoplasmic interaction of LysM receptors contributes to the formation of symbiotic receptor complex.
Auteurs : Maruya Suzuki [Japon] ; Ryota Numazaki [Japon] ; Tomomi Nakagawa [Japon] ; Naoto Shibuya [Japon] ; Hanae Kaku [Japon]Source :
- Plant biotechnology (Tokyo, Japan) [ 1342-4580 ] ; 2020.
Abstract
Receptor complex formation at the cell surface is a key step to initiate downstream signaling but the contribution of this process for the regulation of the direction of downstream responses is not well understood. In the plant-microbe interactions, while CERK1, an Arabidopsis LysM-RLK, mediates chitin-induced immune responses, NFR1, a Lotus homolog of CERK1, regulates the symbiotic process with rhizobial bacteria through the recognition of Nod factors. Concerning the mechanistic insight of the regulation of such apparently opposite biological responses by the structurally related RLKs, Nakagawa et al. previously showed that the addition of YAQ sequence, conserved in NFR1 and other symbiotic LysM-RLKs, to the kinase domain of CERK1 switched downstream responses from defense to symbiosis using a set of chimeric receptors, NFR1-CERK1s. These results indicated that such a subtle difference in the cytoplasmic domain of LysM-RLKs could determine the direction of host responses from defense to symbiosis. On the other hand, it is still not understood how such structural differences in the cytoplasmic domains determine the direction of host responses. We here analyzed the interaction between chimeric NFR1s and NFR5, a partner receptor of NFR1, by co-immunoprecipitation (Co-IP) of these proteins transiently expressed in Nicotiana benthamiana. These results indicated that the cytoplasmic interaction between the LysM-RLKs is important for the symbiotic receptor complex formation and the YAQ containing region of NFR1 contributes to trigger symbiotic signaling through the successful formation of NFR1/NFR5 complex.
DOI: 10.5511/plantbiotechnology.20.0511a
PubMed: 33088201
PubMed Central: PMC7557654
Affiliations:
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Maruya" sort="Suzuki, Maruya" uniqKey="Suzuki M" first="Maruya" last="Suzuki">Maruya Suzuki</name><affiliation wicri:level="1"><nlm:affiliation>Department of Life Sciences, School of Agriculture, Meiji University, 1-1-1 Higashi-Mita, Tama-ku, Kawasaki, Kanagawa 214-8571, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Life Sciences, School of Agriculture, Meiji University, 1-1-1 Higashi-Mita, Tama-ku, Kawasaki, Kanagawa 214-8571</wicri:regionArea><wicri:noRegion>Kanagawa 214-8571</wicri:noRegion></affiliation></author><author><name sortKey="Numazaki, Ryota" sort="Numazaki, Ryota" uniqKey="Numazaki R" first="Ryota" last="Numazaki">Ryota Numazaki</name><affiliation wicri:level="1"><nlm:affiliation>Department of Life Sciences, School of Agriculture, Meiji University, 1-1-1 Higashi-Mita, Tama-ku, Kawasaki, Kanagawa 214-8571, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Life Sciences, School of 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In the plant-microbe interactions, while CERK1, an <i>Arabidopsis</i> LysM-RLK, mediates chitin-induced immune responses, NFR1, a <i>Lotus</i> homolog of CERK1, regulates the symbiotic process with rhizobial bacteria through the recognition of Nod factors. Concerning the mechanistic insight of the regulation of such apparently opposite biological responses by the structurally related RLKs, Nakagawa et al. previously showed that the addition of YAQ sequence, conserved in NFR1 and other symbiotic LysM-RLKs, to the kinase domain of CERK1 switched downstream responses from defense to symbiosis using a set of chimeric receptors, NFR1-CERK1s. These results indicated that such a subtle difference in the cytoplasmic domain of LysM-RLKs could determine the direction of host responses from defense to symbiosis. On the other hand, it is still not understood how such structural differences in the cytoplasmic domains determine the direction of host responses. We here analyzed the interaction between chimeric NFR1s and NFR5, a partner receptor of NFR1, by co-immunoprecipitation (Co-IP) of these proteins transiently expressed in <i>Nicotiana benthamiana</i>. These results indicated that the cytoplasmic interaction between the LysM-RLKs is important for the symbiotic receptor complex formation and the YAQ containing region of NFR1 contributes to trigger symbiotic signaling through the successful formation of NFR1/NFR5 complex.</div></front></TEI><pubmed><MedlineCitation Status="PubMed-not-MEDLINE" Owner="NLM"><PMID Version="1">33088201</PMID><DateRevised><Year>2020</Year><Month>10</Month><Day>23</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">1342-4580</ISSN><JournalIssue CitedMedium="Print"><Volume>37</Volume><Issue>3</Issue><PubDate><Year>2020</Year><Month>Sep</Month><Day>25</Day></PubDate></JournalIssue><Title>Plant biotechnology (Tokyo, Japan)</Title><ISOAbbreviation>Plant Biotechnol (Tokyo)</ISOAbbreviation></Journal><ArticleTitle>Cytoplasmic interaction of LysM receptors contributes to the formation of symbiotic receptor complex.</ArticleTitle><Pagination><MedlinePgn>359-362</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.5511/plantbiotechnology.20.0511a</ELocationID><Abstract><AbstractText>Receptor complex formation at the cell surface is a key step to initiate downstream signaling but the contribution of this process for the regulation of the direction of downstream responses is not well understood. In the plant-microbe interactions, while CERK1, an <i>Arabidopsis</i> LysM-RLK, mediates chitin-induced immune responses, NFR1, a <i>Lotus</i> homolog of CERK1, regulates the symbiotic process with rhizobial bacteria through the recognition of Nod factors. Concerning the mechanistic insight of the regulation of such apparently opposite biological responses by the structurally related RLKs, Nakagawa et al. previously showed that the addition of YAQ sequence, conserved in NFR1 and other symbiotic LysM-RLKs, to the kinase domain of CERK1 switched downstream responses from defense to symbiosis using a set of chimeric receptors, NFR1-CERK1s. These results indicated that such a subtle difference in the cytoplasmic domain of LysM-RLKs could determine the direction of host responses from defense to symbiosis. On the other hand, it is still not understood how such structural differences in the cytoplasmic domains determine the direction of host responses. We here analyzed the interaction between chimeric NFR1s and NFR5, a partner receptor of NFR1, by co-immunoprecipitation (Co-IP) of these proteins transiently expressed in <i>Nicotiana benthamiana</i>. These results indicated that the cytoplasmic interaction between the LysM-RLKs is important for the symbiotic receptor complex formation and the YAQ containing region of NFR1 contributes to trigger symbiotic signaling through the successful formation of NFR1/NFR5 complex.</AbstractText><CopyrightInformation>© 2020 Japanese Society for Plant Biotechnology.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Suzuki</LastName><ForeName>Maruya</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Department of Life Sciences, School of Agriculture, Meiji University, 1-1-1 Higashi-Mita, Tama-ku, Kawasaki, Kanagawa 214-8571, Japan.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Numazaki</LastName><ForeName>Ryota</ForeName><Initials>R</Initials><AffiliationInfo><Affiliation>Department of Life Sciences, School of Agriculture, Meiji University, 1-1-1 Higashi-Mita, Tama-ku, Kawasaki, Kanagawa 214-8571, 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IdType="pubmed">21265894</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Signal Behav. 2018 Feb 1;13(2):e1435228</Citation><ArticleIdList><ArticleId IdType="pubmed">29388878</ArticleId></ArticleIdList></Reference><Reference><Citation>Curr Biol. 2014 Feb 17;24(4):422-7</Citation><ArticleIdList><ArticleId IdType="pubmed">24508172</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Cell Physiol. 2014 Nov;55(11):1864-72</Citation><ArticleIdList><ArticleId IdType="pubmed">25231970</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Cell Physiol. 2019 Aug 1;60(8):1804-1810</Citation><ArticleIdList><ArticleId IdType="pubmed">31119298</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Cell Physiol. 2016 Nov;57(11):2312-2322</Citation><ArticleIdList><ArticleId IdType="pubmed">27565204</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 2007 Dec 4;104(49):19613-8</Citation><ArticleIdList><ArticleId IdType="pubmed">18042724</ArticleId></ArticleIdList></Reference><Reference><Citation>Innate Immun. 2018 Feb;24(2):92-100</Citation><ArticleIdList><ArticleId IdType="pubmed">29105533</ArticleId></ArticleIdList></Reference><Reference><Citation>PLoS One. 2013 Jun 04;8(6):e65055</Citation><ArticleIdList><ArticleId IdType="pubmed">23750228</ArticleId></ArticleIdList></Reference><Reference><Citation>Curr Opin Plant Biol. 2015 Aug;26:64-71</Citation><ArticleIdList><ArticleId IdType="pubmed">26116978</ArticleId></ArticleIdList></Reference><Reference><Citation>Nature. 2003 Oct 9;425(6958):585-92</Citation><ArticleIdList><ArticleId IdType="pubmed">14534578</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed><affiliations><list><country><li>Japon</li></country></list><tree><country name="Japon"><noRegion><name sortKey="Suzuki, Maruya" sort="Suzuki, Maruya" uniqKey="Suzuki M" first="Maruya" last="Suzuki">Maruya Suzuki</name></noRegion><name sortKey="Kaku, Hanae" sort="Kaku, Hanae" uniqKey="Kaku H" first="Hanae" last="Kaku">Hanae Kaku</name><name sortKey="Nakagawa, Tomomi" sort="Nakagawa, Tomomi" uniqKey="Nakagawa T" first="Tomomi" last="Nakagawa">Tomomi Nakagawa</name><name sortKey="Numazaki, Ryota" sort="Numazaki, Ryota" uniqKey="Numazaki R" first="Ryota" last="Numazaki">Ryota Numazaki</name><name sortKey="Shibuya, Naoto" sort="Shibuya, Naoto" uniqKey="Shibuya N" first="Naoto" last="Shibuya">Naoto Shibuya</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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